Multiscale Circuit Analysis
Our ability to perceive and interact with the world around us relies on finely tuned interactions of neural populations across the brain. To understand the functional mechanisms of these interactions, we need to record and manipulate neural activity across multiple scales: from subcellular structures to individual neurons and circuits to networks, which give rise to an organism’s behaviour. Our group is using advanced optical tools such as multicolour in vivo two-photon calcium and closed-loop optogenetics alongside high-resolution tracking of behaving mice and computational analysis to bridge this gap and understand how neural circuits give rise to perception, memory and action.
The focus of our work lies in understanding how the hippocampal memory system integrates sensory and motor information to form long-lasting memories and, importantly, how precisely these memories are influencing behavioural action. For this, we are specifically investigating the neural interactions between hippocampus and hypothalamus, as well as the ventral striatum. We hope that our findings on the circuit mechanisms of memory processing will enable a better understanding of related diseases such as Alzheimer’s and offer potential therapeutic approaches.
Ultimately, we are hoping to identify and describe a complete neural circuit from sensory input to its integration with previous experience to motor action.
Selected Publications
- Barnstedt, O, Mocellin P, Remy S. 2024. A hippocampus-accumbens code guides goal-directed appetitive behavior. Nature Communications, 15: 3196
- Mocellin P, Barnstedt O, Luxem K, Kaneko H, Karpova A, Henschke J, Dalügge D, Fuhrmann F, Pakan J, Kreutz MR, Mikulovic S, Remy S. 2024. A septal-ventral tegmental area circuit drives exploratory behavior. Neuron, 112 (6): 1020-1032.E7
- Felsenberg J, Jacob PF, Walker T, Barnstedt O, Edmondson-Stait AJ, Pleijzier MW, Otto N, Schlegel P, Sharifi N, Perisse E, Smith CS, Lauritzen JS, Costa M, Jefferis GSXE, Bock DD, Waddell S. 2018. Integration of parallel opposing memories underlies memory extinction Cell, 175 (3): 709-722
- Felsenberg J, Barnstedt O, Cognigni P, Lin S, Waddell S. 2017. Re-evaluation of learned information in Drosophila Nature, 544(7649): 240-244
- Žurauskas M, Barnstedt O, Frade-Rodriguez M, Waddell S, Booth MJ. 2017. Rapid adaptive remote focusing microscope for sensing of volumetric neural activity Biomedical optics express, 8(10): 4369-4379
- Perisse E#, Owald D#, Barnstedt O, Talbot CB, Huetteroth W, Waddell S. 2016. Aversive Learning and Appetitive Motivation Toggle Feed-Forward Inhibition in the Drosophila Mushroom Body. Neuron, 90(5): 1086-1099
- Barnstedt O, Owald D, Felsenberg J, Brain R, Moszynski J, Talbot CB, Perrat P, Waddell S. 2016. Memory-Relevant Mushroom Body Output Synapses Are Cholinergic Neuron, 89(6): 1237–1247
Team:
- Dr. Oliver Barnstedt (Group leader)
- Xiaoyu Yang (PostDoc)
- Lam Bui (PhD student)
- Melika Kashizenuzi (MSc Student at OVGU Magdeburg)